Alpha-tubulin 4a Rabbit Polyclonal Antibody

SKU: orb340710

Description

The Alpha-tubulin 4a Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human Alpha-tubulin 4a .Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics, Neuroscience

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1:500-1:2000, IF/ICC: 1:10-1:100
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of human Alpha-tubulin 4a
• Target: TUBA4A
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TUBA1; Tubulin alpha-4A chain; Alpha-tubulin 1; Testis-specific alpha-tubulin; Tubulin H2-alpha; Tubulin alpha-1 chain

Western blot analysis of Alpha-tubulin 4a expression in LOVO (A), HEK293T (B), HUVEC (C) whole cell lysates. (Predicted band size: 49 kD; Observed band size: 52 kD)

Immunofluorescent analysis of Alpha-tubulin 4a staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

TUBA4A

UniProt

Q5XIF6, P68366, P68368

NCBI Gene ID

316531, 7277, 22145