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Anti-Alpha-tubulin (Phospho-Y272) Antibody

Catalog Number: orb665690

DispatchUsually dispatched within 5-10 working days
$ 160.00
Catalog Numberorb665690
CategoryAntibodies
DescriptionRabbit polyclonal antibody to Alpha-tubulin (Phospho-Y272)
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIH, WB
ReactivityHuman, Mouse, Primate, Rat, Zebrafish
ImmunogenKLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y272 of human Alpha-tubulin protein. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1-500-1000
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetTUBA1A
Entrez22142, 22146, 100909441, 84790, 7278, 113457, 7277, 22143, 112714, 10376, 64158, 7846, 500929
UniProt IDQ6AYZ1, P68366, Q13748, Q71U36, P05213, P68373, P68363, P68370, Q6P9V9, Q9BQE3, Q6PEY2, P68369
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesAnti-TUBA1A antibody, anti-TUBA3 antibody, anti-Tu
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-Alpha-tubulin (Phospho-Y272) Antibody

Western blot analysis of Alpha-tubulin (Phospho-Y272) expression in U87MG (A), A375 (B), Hela (C), A2780 (D), EC9706 (E), C6 (F), CT26 (G) whole cell lysates. (Predicted band size: 50; 49 kD; Observed band size: 52 kD)

Anti-Alpha-tubulin (Phospho-Y272) Antibody

Immunohistochemical analysis of Alpha-tubulin (Phospho-Y272) staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.