Tubulin, Alpha 4a Rabbit Polyclonal Antibody

• Catalog Number: orb500742
• Category: Antibodies
• Description: Tubulin, Alpha 4a Rabbit Polyclonal Antibody
• Target: TUBA4A
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human Tubulin, Alpha 4a (41-140/448aa)
• UniProt ID: P68366
• MW: 50 kDa
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: alpha tubulin 1; alpha-tubulin 1; Detyrosinated al
• Research Area: Signal Transduction
• Note: For research use only

Blank control (blue line): Hep G2 (fixed with 70% ethanol (Overnight at 4°C) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-Tubulin, Alpha 4a antibody (orb500742), dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Tubulin, Alpha 4a) Polyclonal Antibody, Unconjugated (orb500742) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-Tubulin, Alpha 4a (orb500742) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Stomach (Mouse) Lysate at 40 ug, Lane 3: Cerebrum (Rat) Lysate at 40 ug, Lane 4: Kidney (Rat) Lysate at 40 ug, Primary: Anti-Tubulin alpha 4a (orb500742) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kD, Observed band size: 50 kD.