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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, ICC, IHC, WB |
|---|---|
| Dilution Range | WB: 3.513888889, IHC: 1:1000-1:5000, ICC: 1:500-1:1000 |
| Reactivity | Bovine, Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Gallus |
| Clonality | Polyclonal |
| Isotype | IgY |
| Immunogen | Recombinant rat alpha-internexin expressed in and purified from E. coli |
| Target | Alpha Internexin/ NF-66 |
| Molecular Weight | 66 |
| Purification | Total IgY fraction |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS + 10 mM NaN3. |
| Concentration | 15 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Immunofluorescence of a section of rat cerebellum showing labeling of axons of granule cell and dendrites of Purkinje with alpha-internexin (Cat. orb319457, 1:5000, red), labeling of the myelin sheaths around the axons with MBP (Cat. orb319582, 1:5000, green), and additional nuclear staining with DAPI (blue).

Immunofluorescence of cultured neurons and glia cells showing specific labeling of neuronal processes with Anti-alpha-internexin (Cat. orb319457, 1:500, red), and specific labeling of microglia with anti-coronin 1a (green).

Western blot of rat cortex lysate showing specific immunolabeling of the ~ 66 kDa alpha internexin protein.
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Protocol Information
Alpha Internexin/ NF-66 Antibody (orb319457)
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