INA (alpha internexin) Antibody (Center)

SKU: orb1930121

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Neuroscience

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:2000, IHC-P - 1:10-50
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB13854
• Immunogen: This INA (alpha internexin) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290-319 amino acids from the Central region of human INA (alpha internexin). Antigen Region: 290-319 aa.
• Target: INA
• Molecular Weight: 55391 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Alpha-internexin, Alpha-Inx, 66 kDa neurofilament protein, NF-66, Neurofilament-66, Neurofilament 5, INA, NEF5

Formalin-fixed and paraffin-embedded human brain tissue reacted with INA antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-INA Antibody (Center) at 1:2000 dilution. Lane 1: Human brain tissue lysate. Lane 2: Mouse brain tissue lysate. Lane 3: Rat brain tissue lysate. Lane 4: SH-SY5Y whole cell lysate. Lane 5: Human liver tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 55 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-INA Antibody (Center) at 1:1000-2000 dilution. Lane 1: Human brain tissue lysate. Lane 2: U-87 MG whole cell lysate. Lane 3: SH-SY5Y whole cell lysate. Lane 4: Mouse liver tissue lysate. Lane 5: Mouse brain tissue lysate. Lane 6: Rat brain tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 55 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

INA

UniProt

Q16352

RefSeq (Protein):NP_116116.1