Alpha Internexin Rabbit Polyclonal Antibody

• Catalog Number: orb1291524
• Category: Antibodies
• Description: Alpha Internexin Rabbit Polyclonal Antibody
• Target: INA
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: Recombinant human Alpha-internexin protein (1-499/499aa)
• UniProt ID: Q16352
• MW: 55 kDa
• Tested applications: ELISA, WB
• Dilution range: WB=1:500-2000, ELISA=1:5000-10000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: a-Internexin; alpha-Internexin; alpha-Internexin;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Measured by its binding ability in a indirect ELISA. Immobilized Human alpha Internexin protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human alpha Internexin Antibody, the EC50 is 312.5 ng/ml.

Measured by its binding ability in a indirect ELISA. Immobilized Human alpha Internexin protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human alpha Internexin Antibody, the EC50 is 911.2 ng/ml.

Sample: Lane 1: Human SH-SY5Y cell lysates, Lane 2: Human SH-SY5Y cell lysates, Primary: Anti-alpha Internexin (orb1291524) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kDa, Observed band size: 66 kDa.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Cerebellum tissue lysates, Lane 3: Rat Cerebrum tissue lysates, Primary: Anti-alpha Internexin (orb1291524) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kDa, Observed band size: 60 kDa.