alpha-Fetoprotein Antibody

SKU: orb43645

Description

Mouse monoclonal antibody to Alpha 1 Fetoprotein

Research Area

Cardiovascular Disease, Epigenetics

Images & Validation

• Tested Applications: ELISA, ICC, IHC-P, RIA, WB
• Reactivity: Human
• Application Notes:
  ELISA: The antibody AFP-11 has been tested as the capture antibody in a sandwich ELISA for analysis of human alpha-fetoprotein in combination with antibody AFP-01 (cat. no. orb43644). Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: hepatocellular carcinoma; heat mediated antigen retrieval (sodium citrate buffer); mAb incubation 1 hour / RT, detection DAB.Western blotting: Recommended dilution: 2 μg/ml, non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: AFP-11
• Immunogen: purified alpha-Fetoprotein
• Target: alpha-Fetoprotein
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AFP, FETA, HPAFP

Immunohistochemistry staining of hepatocellular carcinoma (fetal liver; paraffin-embedded sections) with anti-human alpha-Fetoprotein (AFP-11).

Western blotting analysis of human alpha-fetoprotein using mouse monoclonal antibody AFP-11 on lysates of HepG2 cell line and Jurkat cell line (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody anti-alpha-fetoprotein followed by IRDye800-conjugated anti-mouse secondary antibody. Alpha-fetoprotein was detected at approximately 60 kDa.

Quick Database Links

Gene Symbol

alpha-Fetoprotein

UniProt

P02771

NCBI Gene ID

174