AFP Rabbit Polyclonal Antibody

• Catalog Number: orb1181
• Category: Antibodies
• Description: AFP Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human AFP (501-609/609aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 65 kDa
• Target: AFP
• UniProt ID: P02771
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: alpha Fetoprotein; alpha-fetoprotein; alpha 1 feto
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Mandon, Céline A. et al. Polymer adhesive surface as flexible generic platform for multiplexed assays biochip production Biosens Bioelectron, 39, 37-43 (2013)

PubMed: 22795528

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (AFP) Polyclonal Antibody, Unconjugated (orb1181) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: HepG2 Cell (Human) Lysate at 40 ug, Primary: Anti-AFP (orb1181) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 65 kD, Observed band size: 65 kD.