alpha-Fetoprotein Antibody

SKU: orb43644

Description

Mouse monoclonal antibody to Alpha 1 Fetoprotein

Research Area

Cardiovascular Disease, Epigenetics

Images & Validation

• Tested Applications: ELISA, ICC, IP, WB
• Reactivity: Human
• Application Notes:
  ELISA: The antibody AFP-01 has been tested as the detection antibody in a sandwich ELISA for analysis of human alpha-fetoprotein in combination with antibody AFP-11 (cat. no. 11-384-C100).Western blotting: Recommended dilution: 1-2 μg/ml; non-reducing conditions.Immunoprecipitation: Interaction of the antibody AFP-01 with AFP is dependent on the presence of calcium ions (strongly inhibited by chelating agents). Such characteristics of the antibody can be exploited for immunoaffinity purification of APF under mild elution conditions.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: AFP-01
• Immunogen: purified alpha-Fetoprotein
• Target: alpha-Fetoprotein
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AFP, FETA, HPAFP

Western blotting analysis of human alpha-fetoprotein using mouse monoclonal antibody AFP-01 on lysates of HepG2 cell line and Jurkat cell line (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody anti-alpha-fetoprotein followed by IRDye800-conjugated anti-mouse secondary antibody. Alpha-fetoprotein was detected at approximately 60 kDa.

Quick Database Links

Gene Symbol

alpha-Fetoprotein

UniProt

P02771

NCBI Gene ID

174