4-1BB Antibody

• Catalog Number: orb757397
• Category: Antibodies
• Description: Anti-4-1BB antibody(DM66), Rabbit mAb
• Target: 4-1BB
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Purification: Purified from cell culture supernatant by affinity chromatography
• UniProt ID: Q07011
• Tested applications: ELISA, FC
• Dilution range: ELISA 1/5000-10000|Flow Cyt 1/100
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Antibody Type: Recombinant Antibody
• Clone Number: DM66
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TNFRSF9, 4-1BB, CD137, CDw137, ILA
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human 4-1BB Protein, mFc-His Tag orb689372 can bind Rabbit anti-4-1BB monoclonal antibody (clone: DM66) in a linear range of 1-100 ng/ml.

Expi 293 cell line transfected with irrelevant protein (A) and human 4-1BB (B) were surface stained with Rabbit anti-4-1BB monoclonal antibody 1 μg/ml (clone: DM66) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

Flow cytometry data of serially titrated Rabbit anti-4-1BB monoclonal antibody (clone: DM66) on Expi 293 cell line transfected with human 4-1BB. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Affinity ranking of different Rabbit anti-4-1BB mAb clones by titration of different concentration onto Expi 293 cell line transfected with human 4-1BB. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.