CD137 Antibody (APC)

SKU: orb421964

Description

Mouse monoclonal antibody against CD137 conjugated to APC

Research Area

Epigenetics

Images & Validation

• Tested Applications: FC
• Reactivity: Human, Primate
• Application Notes:
  Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone No.: 4B4-1
• Immunogen: Recombinant human CD137 ectodomain
• Target: CD137
• Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: APC

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Disclaimer: For research use only

Alternative Names

TNFRSF9, 41BB, 4-1BB, MGC2172

Flow cytometry multicolor surface staining of human peripheral blood mononulcear cells stained using anti-humam CD137 (4B4-1) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension) and anti-human CD25 (MEM-181) PE antibody (20 μl reagent per milion cells in 100 μl of cell suspension).

Separation of cells stained using anti-human CD137 (4B4-1) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension, red-filled) from cells stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 1 μg/ml, same as CD137 APC concentration, black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral blood mononuclear cell suspension.

Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononuclear cell suspension stained using anti-humam CD137 (4B4-1) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).

Quick Database Links

Gene Symbol

CD137

UniProt

Q07011

NCBI Gene ID

3604