Alpha-tubulin Mouse Monoclonal Antibody

SKU: orb1294282

Description

Mouse Monoclonal antibody to Alpha Tubulin (2-4D-8D)

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: Western Blot 1:3000-1:6000, Immunofluorescence 1:200–1:500, ELISA 1:10000-1:20000
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: B4X6FX0F
• Target: Alpha-tubulin
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 50mM Tris Glycine, 40% Glycerol (pH7.4). 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

alpha Tubulin monoclonal antibody - 1/3000 dilution, Lysates at 30 µg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294282 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294282 anti-alpha Tubulin monoclonal antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Quick Database Links

Gene Symbol

Alpha-tubulin