α-tubulin Mouse mAb

• Catalog Number: orb763399
• Category: Antibodies
• Description: Mouse monoclonal antibody to alpha-tubulin
• Clonality: Monoclonal
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1mg/mL
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
• Immunogen: Recombinant Protein
• UniProt ID: P68366
• MW: 52kDa
• Tested applications: IF, IHC, IP, WB
• Dilution range: WB 1:5,000-10,000 IP 1:200 IF 1:100-200 IHC 1:200-500
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-alpha tubulin 2 antibody, anti-B alpha 1 anti
• Note: For research use only
• Entrez: 7277

Western blot analysis of 1) Hela, 2) Rat BrianTissue, 3) Mouse Brain Tissue with orb763399 diluted at 1:5, 000.

Immunohistochemical analysis of paraffin-embedded human Colon caricnoma using α tubulin (orb763399) Mouse mAb diluted at 1:500.

1: Input: Mouse Brain Tissue Lysate 2: IP product: IP dilute 1:200 Western blot analysis: primary antibody : orb763399 1:5, 000 Secondary antibody: Goat anti-Mouse IgG, Light chain specific (S003), 1:5, 000.

IF analysis of Hela with orb763399 (Left) and DAPI (Right) diluted at 1:100.