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alpha-Tubulin Antibody

SKU: orb44543

Description

Mouse Monoclonal to alpha tubulin.

Research Area

Cell Biology

Images & Validation

Tested ApplicationsELISA, ICC, IHC-P, IP, WB
ReactivityCanine, Human, Mouse, Plant, Porcine, Rat
Application Notes
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml. Immunoprecipitation: Reducing conditions.Western blotting: Recommended dilution: 1-2 μg/ml. This antibody can be used for Western blotting, but its alternative TU-02 (orb44534) gives better signal in this application.

Key Properties

Antibody TypePrimary Antibody
ClonalityMonoclonal
IsotypeMouse IgM
Clone No.TU-16
ImmunogenPorcine brain microtubule protein MTP-1.
Targetalpha-Tubulin
PurificationPurified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesTris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

TUBA

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
ICC
Immunocytochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
IP
Immunoprecipitation
View Protocol

Available Sizes

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0.1 mg
$ 330.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 5-10 working days.
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