AFP Antibody

SKU: orb1252828

Description

AFP Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Reactivity: Canine, Human, Monkey, Porcine
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 µg/mLIHC(FFPE): 0.5-1 µg/mL for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the AFP antibody to be titered up or down for optimal performance.1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone No.: C3
• Immunogen: Alpha fetoprotein purified from serum of a hepatoma patient was used as the immunogen for this AFP antibody.
• Target: AFP
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AFP Antibody: FETA, HPAFP, Alpha-fetoprotein, Alpha-1-fetoprotein

IHC testing of human fetal liver stained with AFP antibody (C3).

SDS-PAGE Analysis of Purified, BSA-Free AFP Antibody (clone C3). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

Gene Symbol

AFP

UniProt

P02771