AKT3 antibody

• Catalog Number: orb344486
• Category: Antibodies
• Description: AKT3 antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 25F6.F6.D8
• Tested applications: ELISA, FC, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG1
• Immunogen: Anti-AKT3 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT3 protein.
• Concentration: 1.0 mg/ml
• Dilution range: ELISA: 1:2,000 - 1:10,000, FC: User Optimized, IHC: 20 µg/mL, WB: 1:500-1:2000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-AKT3 antibody is directed against human AKT3. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT3 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT3 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT2 and AKT has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q9Y243
• NCBI: NP_001193658.1
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: None
• Alternative names: Mouse anti-AKT3 antibody, AKT-3, PKB antibody, PKB
• Note: For research use only
• Application notes: Anti-AKT3 Antibody is tested in ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT3 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of mouse liver tissue using AKT3 antibody

Western blot analysis of GST Tagged recombinant using AKT3 antibody