Phospho-AKT1+AKT2+AKT3 (Tyr315+316+312) Rabbit Polyclonal Antibody

SKU: orb6789

Description

Phospho-AKT1+AKT2+AKT3 (Tyr315+316+312) Rabbit Polyclonal Antibody

Research Area

Akt/PKB Signaling Pathway, Apoptosis, Cell Cycle, Metabolism, Nuclear Signaling Pathway, PI3K/AKT Signaling Pathway

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human AKT1 around the phosphorylation site of Tyr315 PE(p-Y)LA
• Target: AKT1
• Molecular Weight: 56 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

phospho-AKT1/AKT2/AKT3(Tyr315+Tyr316+Tyr312); AKT1/AKT2/AKT3(phospho-Tyr315/316/312); AKT1/2/3(phospho Y315+Y316+Y312); AKT 1; AKT; AKT1; AKT-1; AKT1_HUMAN; AKT 2; AKT2; AKT-2; AKT2_HUMAN; AKT 3; AKT3; AKT-3; AKT3_HUMAN; C AKT; cAKT; MGC9965; MGC99656; Oncogene AKT1; PKB; PKB alpha; PKB-ALPHA; PKB beta; PKB gamma; PRKBA; Protein Kinase B alpha; Protein kinase B; Proto-oncogene c-Akt; RAC alpha; RAC alpha serine/threonine protein kinase; RAC; RAC PK alpha; Rac protein kinase alpha; RAC Serine/Threonine Protein Kinase; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; v akt murine thymoma viral oncogene homolog 1; vAKT Murine Thymoma Viral Oncogene Homolog 1.

Blank control (blue): EC9706 (fixed with 2% paraformaldehyde for 10 min at 37°C). Primary Antibody: Rabbit Anti-phospho-AKT1+AKT2+AKT3 (Tyr315+316+312) antibody (orb6789, Green), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-AKT1+AKT2+AKT3 (Tyr315+316+312)) Polyclonal Antibody, Unconjugated (orb6789) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-phospho-AKT1+AKT2+AKT3 (Tyr315+316+312) (orb6789) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kD, Observed band size: 63 kD.

Sample: Lane 1: Adrenal glands (Mouse) Lysate at 40 ug, Lane 2: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-phospho-AKT1+AKT2+AKT3 (Tyr315+316+312) (orb6789) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60 kD, Observed band size: 60 kD.

Sample: Lane 1: Rat Cerebrum tissue lysates, Lane 2: Rat Heart tissue lysates, Primary: Anti-phospho-AKT1+AKT2+AKT3 (Tyr315+316+312) (orb6789) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kDa, Observed band size: 65 kDa.

Quick Database Links

Gene Symbol

AKT1

UniProt

P31749