AKT3 Antibody

SKU: orb18912

Description

Goat polyclonal antibody to AKT3

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution Range: ELISA: 1:16000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: A 50-55kDa band observed in Human Hepatoblastoma HepG2 lysates (calculated MW of 55.8kDa according to NP_005456 and 54.0kDa according to NP_859029). Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: AKT3
• Protein Sequence: CSPTSQIDNIGEEEM
• Molecular Weight: 55.8; 54.0
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti AKT3 antibody, anti STK-2 antibody, anti RAC-gamma antibody, anti RAC-PK-gamma antibody, anti DKFZP434N0250 antibody, anti protein kinase B gamma antibody, anti serine threonine protein kinase, Akt-3 antibody, anti RAC-gamma serine/threonine protein kinase antibody

1 µg/ml staining of HepG2 (A) and HeLa (B) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

1 µg/ml staining of Human Thyroid (A), (0.5 ug/ml) Mouse Brain (B) and (1 µg/ml) Rat Brain (C) lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

5 µg/ml staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

5 µg/ml staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunofluorescence analysis of paraformaldehyde fixed THP-1 immobilized on Shi-fix™ plus cover-slips. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor® 488 secondary antibody (1:2000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Anti-Goat IgG followed by Alexa Fluor® 488 secondary antibody.

Immunofluorescence analysis of paraformaldehyde fixed A431. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor® 488 secondary antibody (1:2000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Anti-Goat IgG followed by Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

AKT3

NCBI Gene ID

10000