AKT2 Antibody (S474)

SKU: orb1429337

Description

Rabbit polyclonal antibody to AKT2 (S474).

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Immunology & Inflammation, Immunology & inflammation, Metabolism Research, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF - 1:10-50, WB - 1:1000, IHC-P - 1:10-50
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: Synthetic Peptide
• Target: AKT2
• Molecular Weight: 55769
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RAC-beta serine/threonine-protein kinase, Protein kinase Akt-2, Protein kinase B beta, PKB beta, RAC-PK-beta, AKT2

Fluorescent confocal image of C2C12 cell stained with AKT2 Antibody (S474). C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AKT2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). AKT2 immunoreactivity is localized to Cytoplasm significantly.

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AKT2 Antibody (S474), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of AKT2 (arrow) using rabbit polyclonal AKT2 Antibody (S474). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT2 gene (Lane 2).

Quick Database Links

Gene Symbol

AKT2

UniProt

P31751

RefSeq (Protein):NP_001617.1