Phospho-Akt1 (Thr308) (G12) rabbit mAb

• Catalog Number: orb1946133
• Category: Antibodies
• Description: Phospho-Akt1 (Thr308) (G12) rabbit mAb
• Clonality: Monoclonal
• Clone Number: AktT308-G12
• Tested applications: FC, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Thr308 of human phospho Akt1
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: P31749
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: RAC-alpha serine/threonine-protein kinase, Protein
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of NIH3T3 cell extract untreated or treated with 20% FBS using Phospho-Akt1 (Thr308) antibody AktT308-G12 at 0.1 ug/mL. Cat. #orb1946133.

Western blot analysis of 293T cell extract untreated or treated with 20% FBS using Phospho-Akt1 (Thr308) antibody AktT308-G12 at 0.1 ug/mL. Cat. #orb1946133.

Peptide blocking flow cytometric analysis of K562 cells secondary antibody only negative control (light blue) or treated with LY294002 (red) or with IFNα + IL-4 + pervanadate (green) or LY294002 and blocked with phospho-peptide (black) or IFN + IL4 + Pv and blocked with phospho peptide (gold) or LY294002 and blocked with non-phospho peptide (dark blue) or IFN + IL4 + Pv and blocked with non-phospho peptide (purple) using Phospho-Akt1 (Thr308) antibody AktT308-G12 at 0.5 μg/mL. Cat. #orb1946133.

Flow cytometric analysis of NIH3T3 cells treated with LY294002 + U0126 + wortmanin and stained with secondary antibody as negative control (blue) or treated with LY294002 + U0126 + wortmain (red) or with PDGF (green) and stained using Phospho-Akt1 (Thr308) antibody AktT308-G12 at 0.01 μg/mL. Cat. #orb1946133.

Flow cytometric analysis of C6 cells unstained and treated with imatinib as negative control (blue) or treated with imatinib or with PDGF (green) using Phospho-Akt1 (Thr308) antibody AktT308-G12 at 0.01 μg/mL. Cat. #orb1946133.