Phospho-Akt1 (Ser473) (B9) rabbit mAb

• Catalog Number: orb1946159
• Category: Antibodies
• Description: Phospho-Akt1 (Ser473) (B9) rabbit mAb
• Clonality: Monoclonal
• Isotype: Rabbit IgGk
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Purification: Protein A+G
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser473 of human phospho Akt1
• UniProt ID: P31749
• Tested applications: FC, WB
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Cross Reactivity: Predicted to work with mouse, rat and other homologues.
• Clone Number: AktS473-B9
• Storage: -20°C
• Alternative names: RAC-alpha serine/threonine-protein kinase, Protein
• Note: For research use only

Western blot analysis of NIH3T3 cell extract untreated or treated with PDGF using Phospho-Akt1 (Ser473) antibody AktS473-B9 at 5 ng/mL. Cat. #orb1946159.

Flow cytometric analysis of C2C12 cells secondary antibody only negative control (blue) or 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or untreated (red) or treated with staurosporine (green) using Phospho-Akt1 (Ser473) antibody AktS473-B9 at 0.1 μg/mL. Cat. #orb1946159.

Flow cytometric analysis of Jurkat cells treated with LY294002 (red) or with 20% FBS (green) using Phospho-Akt (Ser473) antibody AKTS473-B9 at 0.05 μg/mL. Cat. #orb1946159.

Western blot analysis of NIH3T3 cells untreated or treated with PDGF using 0.5 ng/mL of Phospho-Akt (Ser473) antibody AktS473-B9 Cat. #orb1946159 or Company C antibody at 60 ng/mL (manufacturer's recommended concentration).