AKT1 + AKT2 + AKT3 Antibody

SKU: orb1537897

Description

AKT1 + AKT2 + AKT3 Antibody

Images & Validation

• Tested Applications: ELISA, FC, ICC, IF, IHC, IHC-P, WB
• Dilution Range: ELISA (1:2000 - 1:10000), Flo, ICC, IF (1:100 - 1:1000), IHC, IHC-P (1:100), WB (1:500 - 1:2000)
• Reactivity: Gallus, Human, Mouse, Rat
• Application Notes:
  Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 1:100.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: AKT Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide R-P-H-F-P-Q-F-S-Y-S-A-S-G-T-A corresponding to the C-terminus (460-480) of human AKT proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling. A BLAST analysis was used to suggest reactivity with this protein from rat, mouse, and chicken based on 100% homology for the immunogen sequence.
• Target: AKT1 + AKT2 + AKT3
• Purification: Delipidated and defibrinated
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
• Concentration: 85 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

AKT1 + AKT2 + AKT3