AKT Mouse mAb

SKU: orb1563196

Description

AKT Mouse mAb

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB: 1/500-1/1000 IP: 1/20
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 3B11-G8-B1
• Immunogen: Purified recombinant human AKT1 protein fragments expressed in E.coli.AKT1 interacts (via the C-terminus) with CCDC88A (via its C-terminus). Interacts with GRB10; the interaction leads to GRB10 phosphorylation thus promoting YWHAE-binding By similarity. Interacts with AGAP2 (isoform 2/PIKE-A); the interaction occurs in the presence of guanine nucleotides. Interacts with AKTIP. Interacts (via PH domain) with MTCP1, TCL1A AND TCL1B. Interacts with CDKN1B; the interaction phosphorylates CDKN1B promoting 14-3-3 binding and cell-cycle progression. Interacts with MAP3K5 and TRAF6. Interacts with BAD, PPP2R5B, STK3 and STK4. Interacts (via PH domain) with SIRT1. Interacts with SRPK2 in a phosphorylation-dependent manner. Interacts with RAF1. Interacts with TRIM13; the interaction ubiquitinates AKT1 leading to its proteasomal degradation. Interacts with TNK2 and CLK2. Interacts (via the C-terminus) with THEM4 (via its C-terminus). Interacts with and phosphorylated by PDPK1.AKT2 interacts (via PH domain) with MTCP1, TCL1A AND TCL1B. Interacts with CLK2, PBH2 and TRAF6. AKT3 interacts (via PH domain) with TCL1A; this enhances AKT3 phosphorylation and activation. Interacts with TRAF6.
• Target: AKT1/AKT2/AKT3
• Molecular Weight: Calculated MW: 56 kDa; Observed MW: 60 kDa
• Purification: Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Concentration: 3 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AKT1

Western blot analysis of total AKT in 3T3, 293T, PC-12 and K562 lysates using AKT (pan) antibody.

Immunoprecipitation analysis of AKT in Hela lysates using AKT (pan) antibody.

Western blot analysis of AKT1, AKT2, AKT3 and DSBA recombinant antigen using DSBA antibody, and (Right) AKT1, AKT2 and AKT3 recombinant antigen fragments using AKT (pan) antibody.

Quick Database Links

Gene Symbol

AKT1/AKT2/AKT3

UniProt

P31749, Q9Y243, P31751