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Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 3
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | Western Blot 1:500-1:1000, Immunofluorescence 1:100-1:200, Immunohistochemistry (Paraffin) 1:50-1:100 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Target | AKT |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered solution, pH 7.2, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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AKT monoclonal antibody at 1/500 dilution. Lysates at 35 µg per lane. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294239 overnight at 4°C.
Immunofluorescence of cell. Sample: HeLa, Green: AKT antibody (orb1294239): 1-100.
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue.
Quick Database Links
Gene Symbol
AKT
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
AKT Mouse Monoclonal Antibody (orb1294239)
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