AKT1 Antibody

• Catalog Number: orb379635
• Category: Antibodies
• Description: Mouse monoclonal antibody to AKT1
• Target: AKT1
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1
• Conjugation: Unconjugated
• Reactivity: Human, Monkey, Mouse, Rat
• Concentration: Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide
• Immunogen: Purified recombinant fragment of human AKT1 (AA: 1-150) expressed in E. Coli.
• UniProt ID: P31749
• MW: 55.7kDa
• Tested applications: FC, ICC, WB
• Dilution range: WB: 1/500 - 1/2000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000
• Antibody Type: Primary Antibody
• Clone Number: 1F7E10
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
• Note: For research use only
• Entrez: 207

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of AKT1 antibody

Flow cytometric analysis of Hela cells using AKT1 antibody

Immunofluorescence analysis of HepG2 cells using AKT1 antibody

Western blot analysis of MCF-7 (Lane 1), NIH/3T3 (Lane 2), Hela (Lane 3), COS7 (Lane 4), and C6 (Lane 5) cell lysate using AKT1 antibody

Western blot analysis of HEK293 cell lysate using AKT1 antibody

Western blot analysis of recombinant protein using AKT1 antibody