AKT2 Antibody

SKU: orb1939407

Description

AKT2 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: WB - 1:500-1:2000
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Molecular Weight: 55769 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

AKT2 antibody western blot analysis in mouse brain tissue lysates (15 μg/lane). This demonstrates the AKT2 antibody detected the AKT2 protein (arrow).

AKT2 Monoclonal Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AKT2 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

All lanes: Anti-AKT2 Antibody at 1:500-1:2000 dilution. Lane 1: Jurkat whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: Mouse brain lysate. Lane 4: C6 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 56 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of AKT2 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).

Quick Database Links

UniProt

P31751