AIF1 antibody

SKU: orb2276291

Description

AIF1 antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Colon: An at least moderate to strong cytoplasmic AIF1 immunostaining should be seen in mucosal macrophages. Negative Control: Colon: AIF1 immunostaining should be absent in all non-inflammatory cells. Cellular Localization: Cytoplasmic and cell surface. Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2c, kappa
• Clone No.: Biorbyt-955M
• Immunogen: Purified recombinant human AIF1 protein (around aa 1-146) (exact sequence is proprietary)

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

Allograft inflammatory factor 1 (AIF1); balloon angioplasty responsive transcription (BART1); IBA1; Interferon responsive transcript 1; Ionized calcium-binding adapter molecule 1; IRT1; Microglia response factor (MRF1)

Duodenum mucosa A strong AIF1 positivity is seen in mucosal macrophages of the duodenum.

Malignant mesothelioma epitheloid subtype containing numerous AIF1 positive inflammatory cells.

Placenta early AIF1 expression is particularly strong in macrophages of the placenta.

Quick Database Links

UniProt

P55008