ADAM12 Antibody

SKU: orb1248028

Description

ADAM12 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Immunohistochemistry:In paraffin embedded Human Prostate shows strong cytoplasm staining of a group of secretory epithelial cells. Recommended concentration: 3-5µg/mL. Paraffin embedded Human Placenta. Recommended concentration: 3.75µg/mL.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and plasma membranes of A431 cells. Recommended concentration: 10µg/mL. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: AARPLPVSPARALC
• Target: ADAM12
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ADAM12, MCMP, MLTN, MLTNA, MCMPMltna, a disintegrin and metalloproteinase domain 12 (meltrin alpha), meltrin alpha, A disintegrin and metalloproteinase domain 12 (Meltrin-alpha, mouse, homolog of), ADAM metallopeptidase domain 12, RP11-295J3.5

orb1248028 (3.8 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1248028 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing cytoplasmic/plasma membrane staining.

orb1248028 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4 ug/ml). IgG control: Unimmunized goat IgG (black line).

Quick Database Links

Gene Symbol

ADAM12

UniProt

O43184

RefSeq (Protein):NP_067673.2, NP_003465.3