ADAM12 Rabbit Polyclonal Antibody (FITC)

• Catalog Number: orb102016
• Category: Antibodies
• Description: ADAM12 Rabbit Polyclonal Antibody (FITC)
• Target: ADAM12
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: FITC
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Rabbit, Rat
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human ADAM12 (201-300/909aa)
• Tested applications: FC
• Dilution range: Flow-Cyt=3μg/Test
• Antibody Type: Primary Antibody
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: A disintegrin and metalloproteinase domain 12; A d
• Research Area: ADAM Protein Family, ADAMs Protein Family, Cancer
• Note: For research use only

Blank control (Black line): U87MG (Black). Primary Antibody (green line): Rabbit Anti-ADAM12 antibody, Dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-FITC. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 10000 events was performed.