ADAM12 Antibody

SKU: orb1256080

Description

ADAM12 Antibody

Research Area

Cancer Biology, Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 589-738 of human ADAM12 (NP_067673.2).
• Target: ADAM12
• Molecular Weight: Observed: 100kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

Adam12, OTTMUSP00000023508, Mltna, mKIAA4001, meltrin-alpha, meltrin alpha, KIAA4001, disintegrin and metalloproteinase domain-containing protein 12, ADAM 12, a disintegrin and metalloproteinase domain 12 (meltrin alpha), a disintegrin and metalloprotease domain 12, a disintegrin and metallopeptidase domain 12 (meltrin alpha), MLTN, UNQ346/PRO545

Western blot analysis of extracts of various cell lines, using ADAM12 antibody (orb1256080) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat heart using ADAM12 antibody (orb1256080) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using ADAM12 antibody (orb1256080) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using ADAM12 antibody (orb1256080) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using ADAM12 antibody (orb1256080) at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

ADAM12

UniProt

O43184