Active Caspase-3 Antibody [H9E5]

• Catalog Number: orb1261318
• Category: Antibodies
• Description: Active Caspase-3 Antibody [H9E5]
• Target: CASP3
• Clonality: Monoclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Immunogen: Recombinant protein of human CASP3
• UniProt ID: P42574
• MW: Observed: 16kDa
• Tested applications: IF, IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
• Antibody Type: Primary Antibody
• Clone Number: [H9E5]
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Caspase-3 Antibody: CPP32, SCA-1, CPP32B, CPP32, C
• Note: For research use only

Western blot analysis of extracts of Jurkat cells, using CASP3 antibody (orb1261318). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of PC-3M cells using CASP3 antibody (orb1261318).

Immunofluorescence analysis of HeLa cells using CASP3 antibody (orb1261318).