H3K27ac Antibody

SKU: orb1256506

Description

H3K27ac Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ChIP, IF, IHC, IP, WB
• Reactivity: Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic acetylated peptide corresponding to residues surrounding K27 of human Histone H3
• Target: H3K27ac
• Molecular Weight: Observed: 16kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

H3F3A, H3t, H3.4, H3/g, H3FT

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K27 antibody (orb1256506) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human mammary cancer using Acetyl-Histone H3-K27 antibody (orb1256506) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using Acetyl-Histone H3-K27 antibody (orb1256506) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H3-K27 antibody (orb1256506) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using Acetyl-Histone H3-K27 antibody (orb1256506) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of C6 cells using Acetyl-Histone H3-K27 antibody (orb1256506) at dilution of 1:100. C6 cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. Blue: DAPI for nuclear staining.

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K27 antibody (orb1256506) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Quick Database Links

Gene Symbol

H3K27ac

UniProt

P68431