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H3C1 Antibody
Description
Research Area
Images & Validation
−| Tested Applications | FC, ICC, IHC-P, WB |
|---|---|
| Dilution Range | WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 ChIP: 1/20 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone No. | R08-4A0 |
| Immunogen | A synthetic acetyl-peptide corresponding to residues surrounding Lys27 of human Histone H3 |
| Target | H3C1 |
| Molecular Weight | Calculated MW: 15 kDa; Observed MW: 15 kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 1xPBS(pH 7.4), 150mM NaCl, 50% Glycerol, 0.02% Sodium azide and 0.05% rAlbumin |
| Concentration | 0.3 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Western blot analysis of Histone H3 (acetylLys27) in Hela, A549, HL-60 lysates using Acetyl-Histone H3 (Lys27) antibody.

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Histone H3 (acetylLys27) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunocytochemistry analysis of Histone H3 (acetylLys27) (green) in hela using Histone H3 (acetylLys27) antibody, and DAPI (blue).
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H3C1 Antibody (orb1564022)
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