Histone H3 K27ac antibody

• Catalog Number: orb420433
• Category: Antibodies
• Description: Histone H3 K27ac antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IP, WB
• Reactivity: C. elegans, Human, Monkey, Mouse
• Isotype: IgG
• Immunogen: Histone H3 [ac Lys27] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide surrounding the K27ac site of human Histone H3.
• Concentration: 0.37 mg/mL
• Dilution range: ELISA: 1: 10,000, IP: 1:100, WB: 1:500-1:1000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Histone H3 [ac Lys27] was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, rat, and C. elegans based on 100% sequence homology. Cross-reactivity with Histone H3 [ac Lys27] from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q71DI3
• NCBI: NP_001005464.1
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Histone H3 Ac Lys27 antibody, Histone
• Note: For research use only
• Application notes: Anti-Histone H3 [ac Lys27] antibody is tested for Dot Blot and Western Blot. This antibody is suitable in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4kDa corresponding to the appropriate cell lysate or extract. Epi-Plus antibody production in collaboration with Novus Biologicals.
• Expiration Date: 12 months from date of receipt.

Dot Blot of Rabbit Histone H3 [ac Lys27] Antibody. Lane 1: K27 unmodified. Lane 2: K27-Ac. Lane 3: K27-Me1. Lane 4: K27-Me2. Lane 5: K27-Me3. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [ac Lys27] antibody at 1:1000 for 45 min at 4°C. Secondary antibody: RABBIT IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-adsorbed at 1:40000 for 30 min at RT. Block: 5% BLOTTO 30 minutes at RT.

Western Blot of Histone H3 [ac Lys27] (RABBIT) Antibody. Western Blot analysis against untreated cell extracts. Lane 2: HeLa cell lysates. Lane 3: NIH/3T3 cell lysates. Lane 4: Cos 7 cell lysates. Lane 5: C. Elegans lysates. Load: 35 µg per lane. Primary antibody: Histone H3 [ac Lys27] antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 15 kDa for Histone H3 [ac Lys27]. Other band(s): non-specific.