ACE2 Antibody

• Catalog Number: orb1239144
• Category: Antibodies
• Description: ACE2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-ACE2 antibody (orb1239144) was raised against a peptide corresponding to 18 amino acids near the carboxy terminus of human ACE2. The immunogen is located within the last 50 amino acids of ACE2.
• Concentration: 1 mg/mL
• Dilution range: WB: 1-2 μg/mL; IHC: 2 μg/mL; IF: 10 μg/mL.Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human, mouse, and rat samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 93kDObserved: 130 kD (7 N-linked glycosylation)
• Target: ACE2
• UniProt ID: Q9BYF1
• NCBI: NP_068576
• Storage: ACE2 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: ACE2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: ACE2 Antibody: ACEH, Angiotensin-converting enzyme
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Independent Antibody Validation (IAV) via Protein Expression Profile in Human Tissues. Loading: 15 µg of lysates per lane. Antibodies: ACE2, orb1239146 (2 µg/mL), ACE2, orb1239144 (2 µg/mL), ACE2, orb1239144 (2 µg/mL) and beta-actin orb1240312 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Tissues and Cell Line. Loading: 15 µg of lysates per lane. Antibodies: ACE2, orb1239144 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: ACE2, orb1239144 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Rat Tissues. Loading: 15 µg of lysates per lane. Antibodies: ACE2, orb1239144 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunofluorescence Validation of ACE2 in Human Testis Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human testis tissue labeling ACE-2 with orb1239144 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunofluorescence Validation of ACE2 in Human Lung Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung tissue labeling ACE-2 with orb1239144 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunofluorescence Validation of ACE2 in Mouse Lung Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung tissue labeling ACE-2 with orb1239144 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunofluorescence Validation of ACE2 in Rat Lung Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat lung tissue labeling ACE-2 with orb1239144 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunohistochemistry Validation of ACE2 in Human Kidney Tissue. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ACE2 antibody (orb1239144) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of ACE2 in Human Kidney Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human kidney cells labeling ACE2 with orb1239144 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Immunofluorescence Validation of ACE2 In Caco2 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Caco2 cells labeling ACE2 with orb1239144 at 5 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). Image showing membrane staining on Caco2 cells.