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ACE2 Antibody

Catalog Number: orb1239135

DispatchUsually dispatched within 5-10 working days
$ 670.00
Catalog Numberorb1239135
CategoryAntibodies
DescriptionACE2 Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, IF, IHC-P, WB
Predicted ReactivityBovine
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenACE2 antibody was raised against a synthetic peptide corresponding to amino acids near the center of human ACE2.The immunogen is located within amino acids 150 - 200 of ACE2.
Antibody TypePrimary Antibody
Concentration1 mg/mL
Form/AppearanceLiquid
ConjugationUnconjugated
MWPredicted: 93kDObserved: 130 kD (7 N-linked glycosylation)
TargetACE2
UniProt IDQ9BYF1
NCBINP_068576
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesACE2 Antibody is supplied in PBS containing 0.02% sodium azide.
Alternative namesACE2 Antibody: ACEH, Angiotensin-converting enzyme
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NoteFor research use only
Application notesWB: 4 μg/mL; IHC: 2 μg/mL; IF: 20 μg/mL.Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human, mouse and rat samples; Immunofluorescence in human, mouse, and rat samples. All other applications and species not yet tested.
Expiration Date12 months from date of receipt.
ACE2 Antibody

Western Blot Validation in Human Tissues. Loading: 15 µg of lysates per lane. Antibodies: ACE2, orb1239135 (4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

ACE2 Antibody

Western Blot Validation in Mouse Stomach Tissue. Loading: 15 µg of lysates per lane. Antibodies: ACE2, orb1239135 (4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

ACE2 Antibody

Western Blot Validation in Rat Brain Tissue. Loading: 15 µg of lysates per lane. Antibodies: ACE2, orb1239135 (4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

ACE2 Antibody

Immunohistochemistry Validation of ACE2 in Human Kidney Tissue. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ACE2 antibody (orb1239135) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

ACE2 Antibody

Immunofluorescence Validation of ACE2 in Human Kidney Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed human kidney cells labeling ACE2 with orb1239135 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).

ACE2 Antibody

Immunofluorescence Validation of ACE2 in Human Testis Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human testis tissue labeling ACE-2 with orb1239135 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

ACE2 Antibody

Immunofluorescence Validation of ACE2 in Human Lung Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung tissue labeling ACE-2 with orb1239135 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

ACE2 Antibody

Immunofluorescence Validation of ACE2 in Mouse Lung Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung tissue labeling ACE-2 with orb1239135 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

ACE2 Antibody

Immunofluorescence Validation of ACE2 in Rat Lung Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat lung tissue labeling ACE-2 with orb1239135 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

ACE2 Antibody

Immunofluorescence Validation of ACE2 In Caco2 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Caco2 cells labeling ACE2 with orb1239135 at 5 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). Image showing membrane staining on Caco2 cells.

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