ACE2 antibody

SKU: orb2276419

Description

ACE2 antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Kidney: Cells of proximal tubuli should show a moderate to strong ACE2 immunostaining with accentuation of the staining at the apical membrane. Negative Control: Kidney: Cells of distal tubuli and glomeruli should not show ACE2 immunostaining. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: Biorbyt-919R
• Immunogen: Recombinant fragment (around aa705-805) of human ACE2 (exact sequence is proprietary)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

Angiotensin I converting enzyme 2

Kidney Clear cell renal cell carcinoma strong strong membraous ACE2 positivity.

Kidney cortex A strong ACE2 immunostaining occurs in proximal tubuli of the kidney.

Testis Leydig cells and spermatocytes of the testis show a particularly strong ACE2 immunostaining in the testis.

Quick Database Links

UniProt

Q9BYF1