EIF4EBP1 Antibody

SKU: orb1240310

Description

EIF4EBP1 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, ICC, IF, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Rat
• Application Notes:
  4E-BP1 antibody can be used for the detection of 4E-BP1 by Western blot at 2.5 - 10 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: 4E-BP1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human 4E-BP1.The immunogen is located within the last 50 amino acids of 4E-BP1.
• Target: EIF4EBP1
• Purification: 4E-BP1 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 4E-BP1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

4E-BP1 Antibody: BP-1, 4EBP1, 4E-BP1, PHAS-I, Eukaryotic translation initiation factor 4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1

Western blot analysis of 4E-BP1 in 3T3 cell lysate with 4E-BP1 antibody at (A) 2.5, (B) 5 and (C) 10 µg/mL.

Immunocytochemistry of 4E-BP1 in 3T3 cells with 4E-BP1 antibody at 2 µg/mL.

Immunofluorescence of 4E-BP1 in 3T3 cells with 4E-BP1 antibody at 20 µg/ml. Green: 4E-BP1 Antibody (orb1240310) Blue: DAPI staining.

Quick Database Links

Gene Symbol

EIF4EBP1

UniProt

Q13541

RefSeq (Protein):NP_004086