Phospho-4E-BP1 (Thr37/46) (A5) rabbit mAb

• Catalog Number: orb1946321
• Category: Antibodies
• Description: Phospho-4E-BP1 (Thr37/46) (A5) rabbit mAb
• Clonality: Monoclonal
• Clone Number: 4EB1T37T46-A5
• Tested applications: FC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Thr37/46 of human phospho 4E-BP1.
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: Q13541
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Eukaryotic translation initiation factor 4E-bindin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of Jurkat cells treated with U0126 (10 uM), 1 hr (red) or treated with TPA 0.2 uM, 30 min (green) using anti Phospho-4E-BP1 (Thr37/46) (A5) rabbit mAb 4EBP1T37T46-A5 #orb1946316 at 1 ug/mL, or concentration-matched rabbit (G9) mAb IgG Isotype Control #orb1946241 for cells treated with U0126 (black) or treated with TPA (blue).

Immunohistochemical staining of rat kidney cells including peptide blocking shows phospho-specificity for 4EB1T37T46-A5 Cat. #orb1946321. Anti-rabbit secondary antibody staining (red) is punctate in the nuclei. Neither control peptide (A) nor non-phospho 4E-BP1 peptide (B) blocks 4EB1T37T46-A5 staining but phospho 4E-BP1 (T37/T46) peptide efficiently blocks signal (C). No signal was observed when the primary antibody is omitted (D). The central object in the field is a glomerulus with surrounding tubules sectioned in various orientations.

Western blot analysis of K562 cell extract untreated or treated with imatinib using Phospho-4E-BP1 (Thr37/Thr46) antibody 4EB1T37T46-A5 at 1 ng/mL. Cat. #orb1946321.

Western blot analysis of NIH3T3 cell extract untreated or treated with PDGF using Phospho-4E-BP1 (Thr37/Thr46) antibody 4EB1T37T46-A5 at 1 ng/mL. Cat. #orb1946321.

Peptide blocking flow cytometric analysis of Jurkat cells LY294002 plus wortmannin plus U0126 LWU) treated cells stained with secondary antibody as negative control (light blue) or treated with LWU (red) or treated with FBS (green) or LUW and blocked with phospho-peptide (black) or FBS and blocked with phospho peptide (gold) or LUW and blocked with non-phospho peptide (dark blue) or FBS and blocked with non-phospho peptide (purple) using Phospho-4E-BP1 (Thr37/Thr46) antibody 4EB1T37T46-A5 at 0.1μg/mL. Cat. #orb1946321.

Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or treated with LY294002 + U0126 + wortmanmin (red) or with FBS (green) using 0.1 ug/mL of Phospho-4E-BP1 (Thr37/Thr46) antibody 4EB1T37T46-A5 (Cat. #orb1946321) or Company C antibody at 0.5 ug/mL (manufacturer's recommended concentration).