4-Hydroxynonenal

SKU: orb1710794

Description

4-Hydroxynonenal (4-HNE) is a key biomarker of lipid peroxidation resulting from oxidative stress. It serves as both a substrate and an inhibitor of the mitochondrial enzyme ALDH2. This small molecule is widely used in research to investigate mechanisms of cellular damage, metabolic disorders, and aging in both in vitro and in vivo models.

Research Area

Metabolism Research

Key Properties

• CAS Number: 75899-68-2
• MW: 156.22
• Purity: 95.12% (May vary between batches)
• Formula: C₉H₁₆O₂
• SMILES: CCCCCC(O)\C=C\C=O
• Target: Dehydrogenase|||Endogenous Metabolite
• Solubility: DMSO:249 mg/mL (1593.91 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:5 mg/mL (32.01 mM)

Bioactivity

• In Vivo:
  Twenty-four hours after fluid percussion injury (FPI), mouse brain tissue is analyzed for the expression levels of NADPH oxidase 1 (NOX1), inducible nitric oxide synthase (iNOS), and 4-Hydroxynonenal (4-HNE). Both wild-type (Nrf2+/+) and Nrf2-deficient (Nrf2-/-) mice show increased 4-HNE expression following a moderate 15 psi injury compared to uninjured controls. In Nrf2-/-knockout mice, 4-HNE expression is significantly higher than in corresponding injured and uninjured Nrf2-/-wild-type animals .
  Read more...
• In Vitro:
  4-Hydroxynonenal (4-HNE) is both a substrate and an inhibitor of ALDH2, with inhibition reversible at low concentrations and becoming irreversible at 10 μM. It induces antioxidant defense mechanisms to limit its own production and enhance cellular protection against oxidative stress. As a product of lipid peroxidation, 4-HNE is mutagenic and genotoxic in viruses, bacteria, and mammalian cells, reacting with all four DNA bases with varying efficiency (G > C > A > T). 4-Hydroxynonenal-dG serves as the best biomarker of its genotoxic effects, primarily found in nuclear DNA .
  Read more...

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Endogenous Metabolite, Cancer, biomarker, 4 Hydroxynonenal, 4-HNE, 4Hydroxynonenal, 4-Hydroxynonenal, Aldehyde Dehydrogenase (ALDH), ALDH2, ALDH, genotoxic, Inhibitor, Human Endogenous Metabolite, EndogenousMetabolite, inhibit, oxidative, nitrosative, mitochondria, stress

Compound Identifiers

PubChem CID

5283344