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Rat 4-Hydroxynonenal (4-HNE) ELISA Kit

Rat 4-Hydroxynonenal (4-HNE) ELISA Kit

Catalog Number: orb1994917

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb1994917
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HNE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HNE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HNE in the samples is then determined by comparing the OD of the samples to the standard curve.
ReactivityRat
Range1.57-100 ng/mL
Assay Time3.5h
Concentration100 ng/mL
Sensitivity0.67 ng/mL
Alternative names4-HNE; HNE; 4-hydroxy-2-nonenal; 4-Hydroxynonenal
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NoteFor research use only
Application notesstandard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HNE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HNE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HNE in the samples is then determined by comparing the OD of the samples to the standard curve
Sample TypesSerum, plasma, tissue homogenates
Expiration DatePlease enquire.
  • Rat 4-HNE ELISA Kit [orb567805]

    Rat

    31.25-2000pg/ml

    18.75 pg/ml

    48 T, 96 T