Mouse 4-Hydroxynonenal (4-HNE) ELISA Kit

• Catalog Number: orb782169
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HNE.
• Tested applications: ELISA
• Reactivity: Mouse
• Range: 2.35-150 ng/mL
• Assay Time: 3.5h
• Concentration: 150 ng/mL
• Sensitivity: 1.1 ng/mL
• Alternative names: 4-HNE, HNE, 4-hydroxy-2-nonenal, 6-Hydroxynonenal
• Note: For research use only
• Application notes: standard: 150 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse 4-HNE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse 4-HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse 4-HNE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: Serum, plasma and other biological fluids.
• Expiration Date: Please enquire.