4 Hydroxynonenal Rabbit Polyclonal Antibody

SKU: orb100588

Featured

Description

Research Area

Amino Acid Metabolism, Oxidative Stress, Vitamins and Minerals

Customer Validated Data

−

The data below is submitted by researchers worldwide. We provide this transparency to help you decide if this product works for your specific application or species, even if we haven't tested it ourselves.

Success by Application

Reactivity Distribution

Latest Experiments

10 Results

Dilution

Sample

Summary

IHC

Rat

1:100

Pancreatic and lung tissues from male Sprague-Dawley rats

Paraffin-embedded tissue sections were deparaffinized, subjected...

Rat

1:1000

Pancreatic and lung tissues from male Sprague-Dawley rats

Sample preparation for western blot involved...

IHC

Mouse

Paraffin-embedded (FFPE) lung sections from mouse

Lung tissues were fixed in 4%...

Mouse

1:2000

Primary mouse microglia

Sample preparation involved lysing cells in...

Mouse

Mouse intervertebral disc (IVD) tissues

Sample preparation involved deparaffinization and rehydration...

1 2 Next

Images & Validation

−

Item 1 of 1

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	All

Related Conjugates & Formulations

−

Key Properties

−

Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated 4-Hydroxynonenal
Molecular Weight	0.156 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

4-Hydroxy-2-Nonenal; 4Hydroxynonenal; 4-Hydroxynonenal; HNE; 4HNE; 4-HNE; (E)-4-Hydroxy-2-nonenal; 2-Nonenal, 4-hydroxy-, (2E)-; (2E)-4-Hydroxy-2-nonenal; 4-Hydroxy-2(E)-nonenal; ; 4-HNE/4-Hydroxynonenal;

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

4 Hydroxynonenal Rabbit Polyclonal Antibody

Sample: Lane 1: 4HNE-BSA (50 ng), Lane 2: BSA (50 ng), Primary: Anti-4 Hydroxynonenal (orb100588) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 0.156 kDa, Observed band size: 66 kDa.

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC-P

Immunohistochemistry Paraffin

View Protocol

IHC-Fr

Immunohistochemistry Frozen

View Protocol

Immunofluorescence

View Protocol

Find More Protocols

Filter by Applications

IHC (1)

Filter by Species

Human (1)

Jie Chu # 1, Jun Jiang # 1 2, Xin Fan # 1 3, Jun Liu 1, Ke Gao 1 4, Yu Jiang 1, Mengxuan Li 1, Wenjin Xi 5, Lu Zhang 1, Ka Bian 6, Angang Yang 7, Rui Zhang A novel MYC-ZNF706-SLC7A11 regulatory circuit contributes to cancer progression and redox balance in human hepatocellular carcinoma Cell Death Differ, (2024)

PubMed: 38862581

Applications

IHC

Reactivity

Human

Fen Zheng 1, Jian-Zhen Lei 2, Jing-Xiao Wang 2, Xiao-Yu Xu 2, Bing Zhou 3, Rui Ge 2, Min Dai 2, Hong-Ke Dong 2, Nan Wu 2, Yue-Hua Li 4, Guo-Qing Zhu 5, Ye-Bo Zhou Crucial roles of asprosin in cisplatin-induced ferroptosis and acute kidney injury Free Radic Biol Med ., (2024)

PubMed: 39653130