4 Hydroxynonenal Rabbit Polyclonal Antibody

SKU: orb100588

Description

4 Hydroxynonenal Rabbit Polyclonal Antibody

Research Area

Amino Acid Metabolism, Oxidative Stress, Vitamins and Minerals

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: All

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated 4-Hydroxynonenal
• Molecular Weight: 0.156 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

4-Hydroxy-2-Nonenal; 4Hydroxynonenal; 4-Hydroxynonenal; HNE; 4HNE; 4-HNE; (E)-4-Hydroxy-2-nonenal; 2-Nonenal, 4-hydroxy-, (2E)-; (2E)-4-Hydroxy-2-nonenal; 4-Hydroxy-2(E)-nonenal; ; 4-HNE/4-Hydroxynonenal;

Sample: Lane 1: 4HNE-BSA (50 ng), Lane 2: BSA (50 ng), Primary: Anti-4 Hydroxynonenal (orb100588) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 0.156 kDa, Observed band size: 66 kDa.

Jie Chu # 1, Jun Jiang # 1 2, Xin Fan # 1 3, Jun Liu 1, Ke Gao 1 4, Yu Jiang 1, Mengxuan Li 1, Wenjin Xi 5, Lu Zhang 1, Ka Bian 6, Angang Yang 7, Rui Zhang A novel MYC-ZNF706-SLC7A11 regulatory circuit contributes to cancer progression and redox balance in human hepatocellular carcinoma Cell Death Differ, (2024)

PubMed: 38862581

Applications

IHC

Reactivity

Human

Fen Zheng 1, Jian-Zhen Lei 2, Jing-Xiao Wang 2, Xiao-Yu Xu 2, Bing Zhou 3, Rui Ge 2, Min Dai 2, Hong-Ke Dong 2, Nan Wu 2, Yue-Hua Li 4, Guo-Qing Zhu 5, Ye-Bo Zhou Crucial roles of asprosin in cisplatin-induced ferroptosis and acute kidney injury Free Radic Biol Med ., (2024)

PubMed: 39653130