tight junction protein 1 Antibody

SKU: orb556544

Description

Rabbit polyclonal antibody to ZO1

Research Area

Epigenetics

Images & Validation

• Tested Applications: WB
• Dilution Range: WB: 1:500-1:3000
• Reactivity: Human
• Application Notes:
  Positive control: HCT116 , 293T , A431 , HeLa

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human ZO-1. The exact sequence is proprietary.
• Target: ZO1
• Molecular Weight: 195
• Purity: Purified by antigen-affinity chromatography.
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Form/Appearance: Liquid: 1XPBS, 1% rAlbumin, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Buffer/Preservatives: PBS, 1% rAlbumin, 20% Glycerol, 0.01% Thimerosal.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

tight junction protein 1 , ZO-1

Western blot analysis of 293T whole cell lysate(Lane 1), A431 whole cell lysate(Lane 2), HeLa whole cell lysate(Lane 3) using ZO1 antibody

Western blot analysis of HCT116 cell using ZO1 antibody

Quick Database Links

Gene Symbol

ZO1

UniProt

Q07157