ZNRF1 Antibody

SKU: orb1248463

Description

ZNRF1 Antibody

Research Area

Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Porcine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 28kDa band observed in Mouse embryo and adult Brain lysates (right panel), consistent with the observed band in transfected N2a cells transiently expressing Znrf1(left panel) Calculated MW of 23.8kDa according to NP_573469.1. Recommended concentration: 1-3µg/mL. Data obtained from Dr. Shuji Wakatsuki, National Institute of Neuroscience, Tokyo, JapanImmunohistochemistry:In paraffin embedded Mouse Cerebral Cortex shows colabeling with Neurofilament M. Recommended concentration, 10µg/mL. Data obtained from Dr. Shuji Wakatsuki, National Institute of Neuroscience, Tokyo, Japan. Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-SDSTYAHGNGYQET
• Target: ZNRF1
• Molecular Weight: Approx 28kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Znrf1, zinc and ring finger 1, B830022L21Rik, MGC101991, Rnf42, Zrfp1, nin283, E3 ubiquitin-protein ligase ZNRF1, nerve injury-induced gene 283 protein, peripheral nerve injury protein nin283, ring finger protein 42, zinc ring finger protein 1, zinc/RING finger protein 1

N2a overexpressing Mouse Znrf1 (mock transfection in first lane) and probed with orb1248463 (1 ug/ml), also staining of Mouse Brain lysates (Embryo E14 and adult cerebellum). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248463 (10 ug/ml) staining of paraffin embedded Mouse Cerebral Cortex. Microwaved antigen retrieval with citrate buffer pH 6, streptavidfine-Alexa 488-staining after biotinylated anti-goat secondary.

orb1248463 (5 ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

ZNRF1

UniProt

Q8ND25

RefSeq (Protein):NP_001162093.1, NP_573469.1