ZNRF1 Antibody

• Catalog Number: orb1248463
• Category: Antibodies
• Description: ZNRF1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Porcine, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-SDSTYAHGNGYQET
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 28kDa band observed in Mouse embryo and adult Brain lysates (right panel), consistent with the observed band in transfected N2a cells transiently expressing Znrf1(left panel) Calculated MW of 23.8kDa according to NP_573469.1. Recommended concentration: 1-3ug/ml. Data obtained from Dr. Shuji Wakatsuki, National Institute of Neuroscience, Tokyo, JapanImmunohistochemistry:In paraffin embedded Mouse Cerebral Cortex shows colabeling with Neurofilament M. Recommended concentration, 10ug/ml. Data obtained from Dr. Shuji Wakatsuki, National Institute of Neuroscience, Tokyo, Japan. Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 28kDa
• Target: ZNRF1
• UniProt ID: Q8ND25
• NCBI: NP_573469.1, NP_001162093.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: Znrf1, zinc and ring finger 1, B830022L21Rik, MGC1
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 28kDa band observed in Mouse embryo and adult Brain lysates (right panel), consistent with the observed band in transfected N2a cells transiently expressing Znrf1(left panel) Calculated MW of 23.8kDa according to NP_573469.1. Recommended concentration: 1-3ug/ml. Data obtained from Dr. Shuji Wakatsuki, National Institute of Neuroscience, Tokyo, JapanImmunohistochemistry:In paraffin embedded Mouse Cerebral Cortex shows colabeling with Neurofilament M. Recommended concentration, 10ug/ml. Data obtained from Dr. Shuji Wakatsuki, National Institute of Neuroscience, Tokyo, Japan. Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

N2a overexpressing Mouse Znrf1 (mock transfection in first lane) and probed with orb1248463 (1 ug/ml), also staining of Mouse Brain lysates (Embryo E14 and adult cerebellum). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248463 (10 ug/ml) staining of paraffin embedded Mouse Cerebral Cortex. Microwaved antigen retrieval with citrate buffer pH 6, streptavidfine-Alexa 488-staining after biotinylated anti-goat secondary.

orb1248463 (5 ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.