ZNRF1 (mouse) Antibody

SKU: orb99058

Description

Goat polyclonal antibody to Znrf1

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Porcine, Rat
• Application Notes:
  IHC: In paraffin embedded Mouse Cerebral Cortex shows colabeling with Neurofilament M. Recommended concentration, 10 μg/ml.WB: Approx 28kDa band observed in Mouse embryo and adult Brain lysates (right panel), consistent with the observed band in transfected N2a cells transiently expressing Znrf1(left panel) Calculated MW of 23.8kDa according to NP_573469.1. Recommended concentration: 1-3 μg/ml

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: ZNRF1 (mouse)
• Molecular Weight: 23.8; 20.2; 14.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration:

  1-3 μg/ml,10 μg/ml

• Disclaimer: For research use only

Alternative Names

anti B830022L21Rik antibody, anti E3 ubiquitin-protein ligase ZNRF1 antibody, anti MGC101991 antibody, anti nerve injury-induced gene 283 protein antibody, anti nin283 antibody, anti peripheral nerve injury protein nin283 antibody, anti ring finger protein 42 antibody, anti Rnf42 antibody, anti zinc and ring finger 1 antibody, anti zinc ring finger protein 1 antibody, anti zinc/RING finger protein 1 antibody, anti Znrf1 antibody, anti Zrfp1 antibody

Western blot analysis of Mouse Brain lysates using Znrf1 antibody

Immunofluorescence analysis of Mouse Cerebral Cortex using Znrf1 antibody

Immunohistochemical staining of Human Cerebellum using Znrf1 antibody.

Quick Database Links

Gene Symbol

ZNRF1 (mouse)

NCBI Gene ID

84937