ZIC3 Antibody

• Catalog Number: orb1264617
• Category: Antibodies
• Description: ZIC3 Antibody
• Target: ZIC3
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
• Immunogen: This ZIC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 239-267 amino acids from the Central region of human ZIC3.
• UniProt ID: O60481
• MW: 51 kDa
• Tested applications: FC, IHC-P, WB
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Zinc finger protein ZIC 3, Zinc finger protein 203
• Research Area: Cell Biology, Neuroscience, Signal Transduction
• Note: For research use only
• NCBI: O60481

Western Blot at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis in CEM cell line lysates (35 ug/lane).

ZIC3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.