ZIC3 Antibody

• Catalog Number: orb1264617
• Category: Antibodies
• Description: ZIC3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This ZIC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 239-267 amino acids from the Central region of human ZIC3.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 51 kDa
• Target: ZIC3
• UniProt ID: O60481
• NCBI: O60481
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Zinc finger protein ZIC 3, Zinc finger protein 203
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis in CEM cell line lysates (35 ug/lane).

ZIC3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.