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Item 1 of 4
ZIC3 Antibody (Center)
Catalog Number: orb1931478
| Catalog Number | orb1931478 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB15116 |
| Tested applications | FC, IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 50569 Da |
| Target | This ZIC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 239-267 amino acids from the Central region of human ZIC3. |
| UniProt ID | O60481 |
| NCBI | NP_003404.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Zinc finger protein ZIC 3, Zinc finger protein 203 Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
ZIC3 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ZIC3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZIC3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
ZIC3 Antibody (Center) western blot analysis in CEM cell line lysates (35 ug/lane). This demonstrates the ZIC3 antibody detected the ZIC3 protein (arrow).
Anti-ZIC3 Antibody (Center) at 1:1000 dilution + HL-60 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 51 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
