ZEB2 Antibody

• Catalog Number: orb1238535
• Category: Antibodies
• Description: ZEB2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: ZEB2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human ZEB2.The immunogen is located within the last 50 amino acids of ZEB2.
• Concentration: 1 mg/mL
• Dilution range: ZEB2 antibody can be used for detection of ZEB2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 134 kDa Observed: 135 kDa
• Target: ZEB2
• UniProt ID: O60315
• NCBI: AAI27103
• Storage: ZEB2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: ZEB2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: ZEB2 Antibody: SIP1, SIP-1, ZFHX1B, HSPC082, SMADI
• Note: For research use only
• Application notes: ZEB2 antibody can be used for detection of ZEB2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ZEB2 in EL4 cell lysate with ZEB2 antibody at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.

Immunocytochemistry of ZEB2 in Jurkat cells with ZEB2 antibody at 20 µg/mL.

Immunofluorescence of ZEB2 in Jurkat cells with ZEB2 antibody at 20 µg/ml. Red: ZEB2 Antibody (orb1238535) Blue: DAPI staining.

Immunofluorescence of ZEB2 in Jurkat cells with ZEB2 antibody at 20 µg/mL.