ZDHHC8 Antibody

• Catalog Number: orb1246768
• Category: Antibodies
• Description: ZDHHC8 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Canine, Mouse, Porcine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-QRDHPQLKTPPSK
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 80-85kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 81.4kDa according to NP_037505.1). Recommended concentration: 1-3ug/ml. An additional band of 48kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ZDHHC8
• UniProt ID: Q9ULC8
• NCBI: NP_037505.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: ZDHHC8, zinc finger, DHHC-type containing 8 , ZDHH
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 80-85kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 81.4kDa according to NP_037505.1). Recommended concentration: 1-3ug/ml. An additional band of 48kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
• Expiration Date: 12 months from date of receipt.

orb1246768 (0.1 ug/ml) staining of Human Brain (Frontal Cortex) lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.