ZDHHC8 Antibody

SKU: orb1246768

Description

ZDHHC8 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Porcine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 80-85kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 81.4kDa according to NP_037505.1). Recommended concentration: 1-3µg/mL. An additional band of 48kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-QRDHPQLKTPPSK
• Target: ZDHHC8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ZDHHC8, zinc finger, DHHC-type containing 8 , ZDHHCL1, ZNF378 , membrane-associated DHHC8 zinc finger protein, zinc finger, DHHC domain containing 8, zinc finger, DHHC domain like containing 1

orb1246768 (0.1 ug/ml) staining of Human Brain (Frontal Cortex) lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

ZDHHC8

UniProt

Q9ULC8

RefSeq (Protein):NP_037505.1